![]() ![]() ![]() Tone: The setting creates a whimsical tone, but the story is full of character and personality.A magical spell turned them into rag dolls, and they must work together to go back to their bodies while saving their relationship. You play as Cody or May, a couple on the verge of divorce. Deep but charming story: The story is the core element of the game.Similar games would have some mix of the elements below: Moreover, dissecting the title will help us identify similar platforming games. Let’s see the elements of the game to better understand its success. Lastly, at the Golden Joystick, it won Best Multiplayer Game. ![]() On Steam, it won the Better With Friends Award. Its trophy rack includes GoTY’s Game of The Year, Best Family Game, and Best Multiplayer. EA and the EA logo are trademarks of Electronic Arts Inc.We’re looking at the top 13 best platforming games like It Takes Two. IT TAKES TWO is a trademark of HAzelight Studios AB. *Friend’s Pass requires installation of the Friend’s Pass Free Trial and one online Friend who owns the game on the same platform and/or next gen platform. Discover innovative and unforgettable gaming experiences, from highly creative game makers who love to enchant and inspire.ĬONDITIONS AND RESTRICTIONS APPLY. In 2018, Hazelight released the BAFTA award-winning A Way Out, the first ever co-op only third person action-adventure, as part of the EA Originals program.ĮA Originals helps shine a light on some of the most passionate, independent, and talented game studios across the globe. Founded in 2014 by Josef Fares, film director and creator of the award-winning game Brothers: A Tale of Two Sons, Hazelight is committed to pushing the creative boundaries of what is possible in games. Hazelight is an award-winning independent game development studio based in Stockholm, Sweden. Join forces, and go on an adventure you’ll treasure – together! Meet a diverse cast of strange and endearing characters. Help Cody and May learn how to overcome their differences. Filled with genre-bending challenges and new character abilities to master in every level, you’ll experience a metaphorical merging of gameplay and narrative that pushes the boundaries of interactive storytelling.Ī UNIVERSAL TALE OF RELATIONSHIPS – Discover a touching and heartfelt story of the challenges in getting along. GLEEFULLY DISRUPTIVE GAMEPLAY – From rampaging vacuum cleaners to suave love gurus – with It Takes Two, you never know what you’re up against next. Choose from couch or online co-op with split-screen play, and face ever-changing challenges where working together is the only way forward. PURE CO-OP PERFECTION – Invite a friend to join for free with Friend’s Pass*, and experience a thrilling adventure built purely for two. They are about to take you on a wild and wondrous ride where only one thing is for certain: we’re better together. It Takes Two is developed by the award-winning studio Hazelight, the industry-leader of cooperative play. Embrace a heartfelt and hilarious story, where narrative and gameplay are interwoven in a uniquely metaphorical experience. Kick gangster squirrels’ furry tails, pilot a pair of underpants, DJ a buzzing night club, and bobsleigh through a magical snow globe. Help each other across an abundance of unexpected obstacles and laugh-out-loud moments. Master unique and connected character abilities in every new level. Together, trapped in a fantastical world where the unpredictable hides around every corner, they are reluctantly challenged with saving their fractured relationship. Play as the clashing couple Cody and May, two humans turned into dolls by a magic spell. Invite a friend to join for free with Friend’s Pass* and work together across a huge variety of gleefully disruptive gameplay challenges. It Takes Two uses Smart Delivery technology - buy the game once and play it on either Xbox One or Xbox Series X|S.Įmbark on the craziest journey of your life in It Takes Two, a genre-bending platform adventure created purely for co-op. *To purchase It Takes Two, please select the Digital Version* ![]()
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![]() “The press release is misleading because it contains statements that are at least potentially deceptive about the availability of the goods,” the judges wrote, according to a copy of the court decision obtained by Bloomberg News. The press release that Apple put out after the December court case is misleading, the court decided, because Apple had been ordered not only to stop selling to iPhone models themselves, but also to recall the iPhones from resellers. The same court ordered Apple to stop making those claims. It’s important to note this isn’t Apple circumventing the ban through clever trickery - instead, it’s doing exactly what Qualcomm wants.Īfter the decision was made by the Munich Regional Court, Apple swiftly shared a press release saying that all iPhone models would continue to be available through resellers and shops of phone carriers at around 4,300 locations in Germany. The ban meant that the affected ranges of iPhones were no longer available for sale anywhere in Germany, and with sales being lower than Apple’s forecasts, it’s possible this lack of sales within a prominent EU nation has forced Apple’s hand. ![]() ![]() German courts cracked down on Apple after it was ruled that Apple was infringing on Qualcomm’s intellectual property. The chip manufacturer won an injunction against Apple in December 2018 that meant it could not sell the affected iPhone ranges within Germany. The ban came about as a result of Qualcomm’s battles with Apple in Germany. ![]() Instead, Apple will begin to resell the affected iPhone models - but with Qualcomm modems installed instead. Apple to only use Qualcomm modems in Germany to get around banĪpple has essentially conceded its German battle with Qualcomm by surrendering its ability to use Intel modems in the iPhone 7, 8, and X in that country. Here’s everything you need to know about the lawsuit battle so far. ![]() ![]() In Japan, the word chairo means the color of tea. In Southeast Asia, the color name often comes from chocolate: coklat in Malay tsokolate in Filipino. Words for the color brown around the world often come from foods or beverages in the eastern Mediterranean, the word for brown often comes from the color of coffee In Turkish, the word for brown is kahve rengi in Greek, kafé, in Macedonian, kafeyev. The current meaning developed in Middle English from the 14th century. Please do not add any text or images directly to this page. The Common Germanic adjective *brûnoz, *brûnâ meant both dark colors and a glistening or shining quality, whence burnish. The first recorded use of brown as a color name in English was in 1000. In politics, it is historically associated with Nazism.The term is from Old English brún, in origin for any dusky or dark shade of color. Culturally, it is most often associated with plainness, humility, the rustic, and poverty. The color is seen widely in nature, in wood, soil, and human hair color, eye color and skin pigmentation. It is a composite color made by combining red, black and yellow. You’re satisfied, press “set as wallpaper.” 8.Brown is the color of dark wood or rich soil. You’ll then be able to move the image to how you like. The makers of Wallpaper Wizard 2 know this only too well, after all, they add 25.000 new images to their collection every month. You’ll then be prompted to select whether you want to set the image as the background of Right corner, click the menu button (three vertical dots). The first image you see here should be the image you downloaded. Open your gallery/photos app and click on the “download” folder.ģ. Search for a wallpaper you like on and download it clicking on the blueĭownload button below the wallpaper. Navigate back to your home screen and take a look at your new wallpaper. Whether you want this image to be set as the background of your lock screen, home screen or both. Here you can arrange the picture how you want it, then tap “set.” 8. Finally, you can spare yourself the pain of sifting through Google Images with Wallpaper Wizard 2, you’ll have enough fresh wallpapers for your Mac’s entire lifetime. (the one that looks like a box with an arrow coming out of it). Choose from hundreds of curated desktop pictures in HD, and the app will set you a new background every week, day, or hour. Navigate to the “Photos” app and find the image you want as your background. Tap on an image and hold on a few seconds. Find an image you like on and click on the blue download button ![]() Exit back to your desktop and see what it looks like! iPhone/iPad 1. Here you’ll want to select your own, so you’ll select the location your new imageĭownloaded. This order, click Apple Menu > System Preferences > Desktop & Screen Saver > Desktop 3. Download your favourite wallpaper clicking on the blue download button below the The folder and click “Set as desktop background.” 6.Enjoy your new wallpaper! Mac 1. That image on your computer (it will probably be in your “downloads” folder) 5.Right-click the image in That part for you.) 3.Click the button, and you’ll notice the image save to your browser. That says “Free Download.” Just below that text is your screen’s resolution (don’t worry, we calculated First, find the perfect wallpaper for your PC. Choose from hundreds of curated desktop pictures in HD, and the app will set you a new background every week, day, or hour. ![]() ![]() ![]() ![]() However, there is also space outside of the frame, says Roberts, and that space is fertile for design and gameplay. It’s like virtual reality though sheer will power.” You forget the room you’re in, you forget the world you’re in, you mentally project yourself through the screen into the world on other side. “We’ve learned to forget that the frame is there. “With the exception of virtual reality, everything we consume is inside a frame, whether it’s the edge of a TV, the edge of a phone, or the edge of a movie screen,” Roberts says. And this is where Roberts found inspiration. The fundamental building block of most comic books – and Gorogoa – is a single, framed image. I had to do something with it, even if it wasn’t a comic.” “This patchwork, or mosaic of framed scenes, is what I found compelling. “It was composing the page that interested me more than telling a sequential story,” Roberts says. Prior to starting work on Gorogoa, Roberts also spent time developing ideas for a comic book, and although the project remains unfinished, the exercise proved useful, and influential. In practice, I think it often goes the other way, and that the process of discovering what story to tell, and what themes to express, is both conscious and unconscious.” “You should decide what story you want to tell, and then decide which mechanics express that story. “I had to find a way to build a story structure with stronger thematic through-lines,” Roberts explains. What it lacked – and what took many years to come together – was its story and themes. ![]() Conceptually, its core elements were in place, but Roberts admits it was not a fully complete articulation of his vision. The response, however, was positive, with players praising the demo’s dreamlike qualities. The first demo for Gorogoa was released in 2012. I ended up throwing out a lot of prematurely polished work, and I maybe even learned more slowly because I think some lessons you can only learn by finishing something.” “I think Gorogoa is not actually a counter example to that, but I think that inexperience added years to the project. “The conventional wisdom is to start with something simple, finish it, move on, do something slightly more ambitious, finish that, and move on.” “You cant skip the learning process when making games,” he says. Beginning his talk, Roberts says the extended development time was unavoidable given his lack of experience. Gorogoa is all the more remarkable for being Roberts’ first game, taking over five years from inception to release. Its achievements were also recognised at this year’s Game Developers Choice Awards, where it won the award for Best Mobile Game. On its release last December, Gorogoa received glowing reviews, with critics praising its intricate design, melancholic atmosphere, and emotional impact. Players manipulate the tiles by moving them around or zooming in and out of their frames.In his talk at GDC 2018, Buried Signal’s Jason Roberts spoke about the themes and complex design process of his meditative point-and-click adventure, Gorogoa. The entire world is contained within four sliding tiles, which interact with each other in surprising and delightful ways. It follows the journey of a protagonist across his lifetime as he chases the titular character and performs enigmatic rituals. Gorogoa’s marvels move across time and space in beautiful and mysterious ways. This won’t feature newly designed puzzles, though rather, it’s the original demo from 2012, which has puzzles that didn’t appear in the final game. Publisher Annapurna has released it on PC, iOS devices, and the Nintendo Switch, and it’s arriving on Xbox One and PlayStation 4 on May 22, which is when the new content will roll out to players across platforms. Jason Roberts’ Gorogoa is one of the most inventive puzzle games to come out in a long time, and the intrigue continues with a new level - sort of. Connect with top gaming leaders in Los Angeles at GamesBeat Summit 2023 this May 22-23. ![]() ![]() ![]() Print directly within your QuickTime movie Wired actions allow a QuickTime movie to modify and control itself (and other QuickTime movies) via scripts embedded within the movie. Totally Hips QScript Extension Essentials (QSXE) component allows you to utilize custom wired actions inside your QuickTime movies. Includes Synchromy nondestructive color conversion. Supports all standard uncompressed formats: RGB, YCbCr 4:4:4, 4:2:2 optional alpha 10bit, 8bit progressive, interlaced SD, HD, anyD NTSC, PAL 4:3, 16:9, any:any arbitrary frame rate. For uncompressed formats, SheerVideo doubles disk speed, disk capacity, and transmission bandwidth with perfect fidelity, bit-for-bit identical to the original. SheerVideo is a powerful, faster-than-real-time nondestructive codec for production, postproduction, and archival. ![]() Now available for both Mac OS X and Windows. With FBX for QuickTime, digital artists can share, view, and interact with 3D content seamlessly, efficiently, and at no cost. The codec works with any application that supports broadcasting or editing in an Apple QuickTime environment.Īutodesks FBX for QuickTime enables QuickTime as a 3D viewing solution that supports content from any professional 3D package. The combination of Apple QuickTime and ACT-元 provides a low cost, high quality, high compression solution ideal for electronic field production. The ACT-元 codec enables users to bring ACT-元 into an Apple QuickTime environment. The ZyGoVideo component adds encoding capabilities to QuickTime Pro. ZyGoVideo provides high-quality video at prevalent modem rates. ZyGoVideos great looking, smooth playing, web-video codec is now available for Windows, Mac PPC and Mac Intel machines. Xvid is a video codec library following the MPEG-4 standard. The Xvid component for QuickTime allows users to play & encode Xvids in QuickTime Player. The latest DivX Codec ( Win or Mac) lets you play DivX video in QuickTime Player and also consists of improved tools for the utmost encoding quality and performance, making it the best choice to convert or create your own DivX video content. Version 7.1.6 or higher is required to playback media with closed captions. QuickTime Player supports standard CEA-608 closed captions. PowerPC-based components will not work in QuickTime on Macintosh with Intel processors. Note: If you are using a Macintosh with an Intel processor be sure to visit the component developer's web site to download a Universal Binary Component that is compatible with your Intel-based Macintosh. If you encounter media that requires the installation of a third-party QuickTime component, please follow the link below to the third-party developers web site to see if it is available for download. These third-party components expand the functionality of QuickTime, allowing QuickTime to play additional media types. With QuickTimes open architecture, third-party developers can create components, or plug-ins to QuickTime. Welcome to the QuickTime third-party component page. ![]() ![]() ^ 3 Waterval was released on DecemKids Mode ^ 2 Princess was released on November 18, 2021 ^ 1 My Way was released on November 9, 2021 Note: The following songs were only added to the game after the release date of November 4, 2021: There are 46 tracks in this game, including 5 regional exclusives. A (S) indicates this map is exclusive to the Southeast Asian countries of Indonesia, Malaysia, the Philippines, Singapore, Thailand, and Vietnam, as well as Hong Kong and Taiwan.A (J) indicates this map is exclusive to Japan.A (G) indicates this map is exclusive to Germany.An (FR) indicates this map is exclusive to France.A (CA) indicates this map is exclusive to Canada.A (BL) indicates this map is exclusive to the Benelux region. ![]() An (XK) indicates this map is not available in the Korean version of the game.Maps in this category were absent on early access copies of the game until Novemunless otherwise noted. An (I) indicates that this map requires an internet connection to be played.An asterisk (*) indicates this song is covered in-game.Due to the omission of the sticker album, stickers are no longer offered through the Gift Machine. Additionally, the Gift Machine now appears in a purple galaxy print design and rewards players with avatars, aliases and skins. However, the sticker album is not present this time. Likewise, the Profile tab from Just Dance 2021 has returned. ![]() The pixelated texture has been removed and the colored parts now appear in solid colors instead of gradients. The menu is very similar in appearance to Just Dance 2021. Use the free Just Dance Controller App, available on iOS 10 and Android 5.0, to play the game on any platform and track scores for up to six players without any additional accessories needed. Kids Mode: Eight routines made specifically for kids.Sweat Mode: Start your own routine and keep yourself motivated by tracking calories burnt and time spent dancing.You will be matched with players of a similar level as yours for a fair dance battle. World Dance Floor: Challenge fellow Just Dancers from all over the world in these exciting tournaments.Co-op Mode: Team up with friends to combine scores to rule the dance floor. ![]() ![]() ![]() Top 6 Desk Organizers for a Clutter-Free Office Setup It’s easy to make a mess on your office desk with a pencil, pen, sticky notes, highlighter, paper clips, and other stationery items. Top 5 Desk Pads for Your Ideal Work From Home Setup Your ideal work from home setup deserves a desk pad to keep the keyboard, mouse, speaker, and other accessories. ![]() Top 7 Coffee Mug Warmers for Your Office Desk Imagine this: you are sorting through your emails on a busy Monday morning. Top 5 Desk Exercise Bikes You Can Find on Amazon An average American walks somewhere between 1.5 to 2 miles daily. 6 Best Upright Vacuum Cleaners for Cleaning Pet Hair and Carpets Cleaning after pet hair at home can be tiring. What Are PC Cleaning Tools and Should You Use Them "Has your PC become slow?""A scan detected 3049 issues with your Windows computer. More importantly, do wash your hands thoroughly after cleaning your products. Hence, it’s of utmost importance to use the right cleaner and the right method for cleaning and sanitizing your gadgets. Hello, FreshnessĮlectronics can be delicate, and some of them may require a little delicate handling than others when cleaning. Pro Tip: You shouldn’t wash microfiber cloth or dusters with fabric softeners. So when it has gathered enough dust and dirt, just twist the head and throw it in the washing machine. The best part is that the head is washable. It’s small and compact, which is a huge plus, especially from the storage point of view. ![]() The cleaning head is made of microfiber strands to trap dust and lint fast and quickly. So on days that you just want your desk to be just dust-free, you can use the Oxo duster to do your bidding. Plus, deep cleaning requires time and effort and is not an everyday affair. Not everything requires a complex set of tools and gadgets to be kept clean. And most cleaning solutions fail to take care of this issue. Marianna 99% Isopropyl AlcoholĪpart from dust, lint, and pet hairs (if you have a pet), the frequently-touched accessories on your desk can also house germs and bacteria. Pro Tip: Avoid using tissues and paper towels to clean screens, however soft they may be, since the rough edge may scratch the surface. All you need to remember is to spray the liquid on the cloth first and then wipe the monitor.Īpart from monitors, you can use this cleaner to wipe the screen of your tablet, smartphone, and even your reading glasses.ĭo note that spraying cleaning liquid or other substances directly into the screen (be it a TV or a monitor) may cause the liquid to seep into the display and cause discoloration of the screen. The company includes a microfiber cloth for easy cleaning. It leaves no streak marks, and that’s an added plus. Dedicated screen cleaners like the one from Screen Mom are a simple one-step solution to keep your screen clean from dust, smudges, and greasy stains. Computer monitors require a little more care than the humble desk, as the wrong liquid can destroy the anti-reflecting coating. ![]() ![]() ![]() You can also upload your own voice clip onto the study sets and Slide Activities with Audio Clip Slide App! This feature is exclusive to our Pro plan users. If you would like to your students to upload voice clips to you, you can do it with the Voice Slide App. If you set the language to 'Others', then the text will not be read. In a written report, we can easily identify the executive summary and quickly see the outline at a glance. This example shows both the term and definition language in French. One of the best ways to keep your audience’s attention is to structure your speech with signposts or transitions which tell your audience where they are and where they are going. Upload your own voice clip if you need this to be read. Only the Chinese characters will be read. They are used to alert audiences to the fact that you are getting ready to discuss something else. Note: In Chinese, the 'hanyupinyin' will not be read. Definition 1 / 70 These are phrases or sentences that lead from one distinct-but-connected idea to another. ![]() Example below shows the term in Chinese and the definition in English: Signposting language can help you guide the reader through your writing and make sure the order is clear and flows well. Default language is English.Ĭhange the language via the drop-down list within the study set. But yet again, this refers to the licensing of. Supported Text-to-Speech languages are English, French, German, Spanish, Chinese and Malay / Indonesian. A common definition of Free Content and Free Software is that it is free as in free speech, not free beer. Immersive Reader capabilities are found in the Signpost and Slide Activities. Signposts can be displayed as a physical change of direction or a deliberate movement of the speaker. So in other words, it acts as a guide so the listener can follow what you are saying. It is used to make clear what has just happened, and what is going to happen next. Have students with read-aloud accommodations? We got you covered with our Text-To-Speech functions in Deck.Toys! It is currently enabled for text in Study Set Activity and Locks. A signpost is a verbal statement or visual cue used by the speaker to guide or engage the audience while bringing them through the various stages of a speech or presentation. Signposting language is the words and phrases that people use in order to guide the listener coherently through what is being said. How Do I Configure Text-to-Speech and Audio Clips? ![]() ![]() ![]() The filming session of the video for the single took place during January 2002. It would later get ranked the second best performing single of 2002, and listed as the 132nd best single. "Foolish" spent 10 consecutive weeks at number one on the Billboard charts and a total of 17 weeks in the top 10. It follows a chord progression of F maj7–G, and Ashanti's vocals span from A 3 to C 6. The song is performed in the key of C major in common time with a tempo of 89 beats per minute. In 2009, Billboard ranked it 19th on its Hot 100 Songs of the 2000s Decade. The song was nominated for Best Female R&B Vocal Performance at the 2003 Grammy Awards, and won the Soul Train Music Award for Best R&B/Soul Single – Female. Elsewhere, "Foolish" became a top ten hit in the United Kingdom, Australia, New Zealand and Canada. It spent ten weeks at number one on the US Billboard Hot 100 and on the Hot R&B/Hip-Hop Songs chart, becoming Ashanti's second number one and third top ten on both charts. The song was released as the album's lead single on February 11, 2002. A promotional version of the single called "Unfoolish" features a verse from The Notorious B.I.G. Due to the inclusion of the sample, Mark DeBarge and Etterlene Jordan are also credited as songwriters. The song heavily samples DeBarge's " Stay with Me". It was written by Ashanti, Etterlene Jordan, Mark DeBarge and Irv Gotti, while production was overseen by Gotti. It served as her debut single, the first from her self-titled debut album (2002) and was released by Def Jam Recordings, AJM, and Irv Gotti's Murder Inc. Looking out my window, knowing that I should goĮven when I pack my bags, this something always hold me back The easy, fast & fun way to learn how to sing: 30DaySinger." Foolish" is a song by American singer-songwriter Ashanti. So sad, so sad what love will make you doīaby, why you hurt me, leave me and desert me?īoy, I gave you all my heart and all you did was tear it up I can't see how you could bring me to so many tears after all these years See when I'm home, I'm all alone and you are always goneĪnd boy, you know I really love you, I can't deny I don't know how I allow you to treat me this way and still I stayīaby, I don't know why you wanna do me wrong You said you love me, no one above me and I was all you hadĪnd though my heart is beating for you, I can't stop crying Struggling with Foolish? Become a better singer in 30 days with these videos! Murder Inc.īaby, I don't know why you're treating me so bad ![]() Elsewhere, "Foolish" became a top ten hit in the United Kingdom, Australia, New Zealand, Canada, Germany and Japan. The song was released as the album's lead single on January 29, 2002. A promotional version of the single called "Unfoolish" features a verse from Notorious B. The song uses a sample of DeBarge's "Stay with Me" and features elements from the remix of "One More Chance" by The Notorious B. It was written by Ashanti, 7 Aurelius, Etterlene Jordan, Mark DeBarge and Irv Gotti, while production was overseen by Gotti. It served as her debut single, the first from her self-titled debut album (2002). "Foolish" is a song by American singer-songwriter Ashanti. ![]() ![]() ![]() Therefore, diverting carbon allocation from sugars to lipids, in HL plants, likely alters other metabolisms in the plant cells. The intracellular availability of sugars is well recognized for its roles in communication between sugar sensing and redox chemistry. Throughout the day/night cycle, plants breakdown assimilated carbon through respiration for growth and maintenance. ĭuring the day, plants fix inorganic CO 2 by carboxylating ribulose-bisphosphate, producing phosphorylated intermediates which is subsequently partitioning into several central metabolic pathways, including the generation of carbohydrates, proteins and lipids. speculated the trade-off between lipids and sugars in HL leaves might alter the leaf sugar homeostasis, which in turn mitigates negative feedback on photosynthesis. To explain this secondary growth benefit, Beechey-Gradwell et al. These plants had up to twice the FA content in mature leaves (from ~ 3.5% to ~ 7% dry weight (DW)) as well as increased plant CO 2 assimilation rate and biomass. We previously reported the development of transgenic lines of high-lipid (HL) perennial ryegrass ( Lolium perenne) and Arabidopsis, that co-express diacylglycerol O-acyltransferase (DGAT, the enzyme for TAG biosynthesis) and cysteine-oleosin (Cys-OLE, a modified oleosin designed to protect lipid droplets from proteolysis). Increased TAG synthesis in vegetative tissues can be achieved by promoting the carbon flux into fatty acid (FA) and TAG synthesis through the manipulation of enzymes and/or transcriptional regulators whilst suppressing FA catabolism, including FA utilization and TAG turnover. To address this, we and others have been exploring biotechnological approaches to increase the levels of energy-rich triacylglycerol (TAG) content in plant non-seed tissues. The climate crisis and increasing world population mean dramatic increases in the production of food, forage and renewable fuels is needed using the existing agricultural land. Further inquiries can be directed to the corresponding author/s.įunding: This manuscript was funded by AgResearch Science Prize 2017 and the New Zealand Ministry of Business, Innovation and Employment through the research programme (C10X1603).Ĭompeting interests: The authors have declared that no competing interests exist. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: The minimal dataset is contained in the paper and Supporting information files. ![]() Received: JAccepted: SeptemPublished: October 13, 2022Ĭopyright: © 2022 Winichayakul et al. PLoS ONE 17(10):Įditor: Jong-Seong Jeon, Kyung Hee Univeristy, REPUBLIC OF KOREA (2022) Insight into the regulatory networks underlying the high lipid perennial ryegrass growth under different irradiances. ![]() Overall, this study emphasizes the importance of carbon and energy homeostatic regulatory mechanisms to overall productivity of the HL Lolium through photosynthesis, most of which are significantly impacted by low irradiances.Ĭitation: Winichayakul S, Macknight R, Le Lievre L, Beechey-Gradwell Z, Lee R, Cooney L, et al. Many of the above upregulated or downregulated transcript levels were found to be complemented by growing the plants under low light. The plants also had altered several transcripts involved in mitochondrial oxidative respiration and redox potential. ![]() Under standard lights, analysis of differentiating transcripts in HL Lolium reveals that the plants had elevated transcripts involved in lipid metabolism, light capturing, photosynthesis, and sugar signalling while reduced expression of genes participating in sugar biosynthesis and transportation. In this study, we demonstrated that the growth advantage was not displayed in HL Lolium grown in spaced pots under low lights. Besides, the underlying genetic regulatory network linking between leaf lipid sinks and these physiological changes remains unknown. The greater aboveground biomass was found to be diminished in a dense canopy compared to spaced pots. Under favourable conditions, perennial ryegrass ( Lolium perenne) engineered to accumulated high lipid (HL) carbon sink in their leaves was previously shown to also enhance photosynthesis and growth. ![]() |
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